Correlative Microscopy Techniques

Correlative Microscopy Techniques

  • The modular design of the WITec microscope systems allows to combine various imaging techniques such as Raman imaging, fluorescence, luminescence, atomic force microscopy (AFM), near-field microscopy (SNOM or NSOM) and scanning electron microscopy in one single instrument without compromising the analyzing and imaging quality.

    The integrated WITec software interface enables user-friendly measurement control, quick and easy correlation of the results and image overlay.

  • Correlative Raman-fluorescence microscopy on a biological cell. Green: GFP fluorescence; yellow, pink and white: Raman signal of nucleus, nucleoli and lipids, respectively.

Popular Correlative Techniques

  • Raman-SEM

    The Raman-SEM (RISE) combination is a unique correlative microscopy technique provided by WITec. Structures in the nm-range detected by Scanning Electron Microscopy (SEM)  can be correlated to chemical Raman imaging from the same sample area.

    More information about WITec's RISE Microscope

  • Correlative Raman-SEM (RISE) image of TiO2. The Raman image is overlaid on the SEM image. Red and blue: Different modifications of TiO2 analyzed by Raman microscopy.
  • Raman-AFM

    By combining confocal Raman imaging with AFM, the chemical composition of the sample can be linked with the surface characteristics. These two complementary techniques are available in combined Raman-AFM WITec microscopes for flexible and comprehensive sample characterization.

    More information about WITec's Raman-AFM Microscope alpha300 RA

  • Correlative Raman-AFM image of a Polymer Blend. Left: Topographic AFM image. Right: Raman image. Red, blue and green: different polymers of the blend.
  • Correlative AFM-Raman-SNOM-Photoluminescence Microscopy of CVD-grown WS2 crystals.